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KMID : 0545120130230040555
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 4 p.555 ~ p.559
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A Novel Marker for the Species-Specific Detection and Quantitation of Vibrio cholerae by Targeting an Outer Membrane Lipoprotein lolB Gene
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Cho Min-Seok
Ahn Tae-Young
Joh Ki-Seong
Paik Soon-Young
Kwon Oh-Sang
Jheong Won-Hwa
Joung Yo-Chan
Park Dong-Suk
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Abstract
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Vibrio cholerae O1 and O139 are the major serotypes associated with illness, and some V. cholera non-O1 and non-O139 isolates produce cholera toxin. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the species-specific detection and quantitation of V. cholera using a primer pair based on an outer membrane lipoprotein lolB gene for the amplification of a 195 bp DNA fragment. The qPCR primer set for the accurate diagnosis of V. cholera was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.
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KEYWORD
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Detection, Diagnosis, Quantitation, outer membrane lipoprotein LolB, Vibrio cholera
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